2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.
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Incubate the cores for 1. If you wish to download it, please recommend it to your friends in any social system. Periodically swirl the containers.
Osmosis diffusion of water through a selectively permeable membrane Membrane X is permeable to water but not to protein Which side has the highest concentration.
Incubate the cores for at least 1. Do the cores show various degrees of turgor? Alternately, for a more accurate measurement of changes in the solution density, a refractometer can be used. We think you have liked this presentation. Change in Weight final – initial g.
Use a cork borer to prepare at least 27 uniform tissue samples from the potato. About project SlidePlayer Terms of Service. Measuring Water Potential by the Gravimetric Technique. The answer will be in Jm -3 which are equivalent to chaddakov Pa. Registration Forgot your password?
This technique cbardakov measuring water potential is similar in theory to the Chardakov method and shares the advantage of being simple to perform and doesn’t require expensive equipment. Draw the best fit line for your data. The Chardokov method provides a quick means to determine plant tissue water potentials.
An alternate method to determine his point requires performing a regression analysis of the best fit line of your data. Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel.
A measure of how much material is packed chrdakov a unit volume of the material The fewer. Change in weight of potato cores incubated in sucrose solutions. OK Any substance that can change shape or charrdakov easily. My presentations Profile Feedback Log out. Then, the percent change in weight of the tissue is plotted versus solution concentration or osmotic potential. In both techniques, tissue samples are incubated in a mmethod of solutions of known osmotic water potential.
Dispense 10 mL of water or sucrose 0. Incubating tissue in solution Pre-incubationIncubation Post-incubation Save for later measurement.
Investigating osmosis: measuring the water potential of a potato cell
Repeat for all solutions. In this method, tissue samples are methodd before and after incubation in a series of solutions of known osmotic water potential. This increases the tissue weight and may lead to small errors.
If the density of a solution does not change no net movement of water then this solution has the same methdo potential as the tissues that were incubated in it. What is the water potential of potato tissue? Pour off the solutions into a set of empty, correspondingly labeled tubes. Boy, Oh Buoyancy Does it Float?
Then remove the tissues, gently blot on paper towels and reweigh. Chardakov Method for Determining Water Potential. Use a cork borer to prepare at least 27 uniform tissue samples from the potato. Dispense 10 mL of water or a sucrose solution 0. What is the water potential of potato tissue? Share buttons are a little bit lower. This is due to incipient plasmolysis that occurs at low solution water potential. Two ways to move water Bulk flow Osmosis Both move water from high energy to low Differences —Source of energy difference.
A “kink” may be observed in the graph below the x-axis. Which is more accurate? From the graph, determine the concentration of the sucrose solution in which there was no net weight gain i. Weigh two or preferably three cores, record your data in Table 2 and then place the cores in one of the test solutions.
No change in density, concentration No net water movement Tissue and solution were at the same WP If we can find metohd solution concentration —We can find the tissue WP. This occurs when the apoplast becomes infiltrated with water and solutes.